Migration:
Suspension:Article Title: Endophytic commensal bacteria capitalize on the AvrPto-FER pathway to enhance proliferation during early stages of pathogen invasion.
Article Snippet: .. The bacterial suspension was adjusted to OD600 = 0.1, and Col-0 seedlings were incubated in the bacterial suspension for 8 h. The seedlings were ground and subjected to protein extraction in extraction buffer (20 mM HEPES pH 7.5, 150 mM NaCl, 10% [v/v] glycerol, 1% [v/v] Triton X-100, 5 mM EDTA, 10 mM DTT, 1 mM PMSF, and Halt Protease Inhibitor Cocktail [Thermo Fisher Scientific, USA]) for 1 h. The samples were centrifuged at 12,000 g for 10 min at 4°C, and the supernatants were collected. .. The samples were run on a 10% SDS-PAGE gel and analyzed by immunoblotting with anti-FER and anti-FERpY648 antibodies (ABclonal, China).
Article Title: Endophytic commensal bacteria capitalize on the AvrPto-FER pathway to enhance proliferation during early stages of pathogen invasion
Article Snippet: .. The bacterial suspension was adjusted to OD 600 = 0.1 and Col-0 seedlings were incubated in the bacterial suspension for 8 h. The seedlings were ground and subjected to protein extraction in extraction buffer (20 mM HEPES pH 7.5, 150 mM NaCl, 10% [v/v] glycerol, 1% [v/v] Triton X-100, 5 mM EDTA, 10 mM DTT, 1 mM phenylmethylsulfonyl fluoride (PMSF), and halt protease inhibitor cocktail [Thermo Fisher Scientific, USA]) for 1 h. The samples were centrifuged at 12 000 g for 10 min at 4°C, and the supernatants were collected. .. The proteins were fractionated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and the phosphorylated proteins were analyzed by immunoblotting with anti-phosphoserine (anti-Ser) and anti-phosphothreonine (anti-Thr) antibodies (Abcam, USA).
Incubation:Article Title: Endophytic commensal bacteria capitalize on the AvrPto-FER pathway to enhance proliferation during early stages of pathogen invasion.
Article Snippet: .. The bacterial suspension was adjusted to OD600 = 0.1, and Col-0 seedlings were incubated in the bacterial suspension for 8 h. The seedlings were ground and subjected to protein extraction in extraction buffer (20 mM HEPES pH 7.5, 150 mM NaCl, 10% [v/v] glycerol, 1% [v/v] Triton X-100, 5 mM EDTA, 10 mM DTT, 1 mM PMSF, and Halt Protease Inhibitor Cocktail [Thermo Fisher Scientific, USA]) for 1 h. The samples were centrifuged at 12,000 g for 10 min at 4°C, and the supernatants were collected. .. The samples were run on a 10% SDS-PAGE gel and analyzed by immunoblotting with anti-FER and anti-FERpY648 antibodies (ABclonal, China).
Article Title: Endophytic commensal bacteria capitalize on the AvrPto-FER pathway to enhance proliferation during early stages of pathogen invasion
Article Snippet: .. The bacterial suspension was adjusted to OD 600 = 0.1 and Col-0 seedlings were incubated in the bacterial suspension for 8 h. The seedlings were ground and subjected to protein extraction in extraction buffer (20 mM HEPES pH 7.5, 150 mM NaCl, 10% [v/v] glycerol, 1% [v/v] Triton X-100, 5 mM EDTA, 10 mM DTT, 1 mM phenylmethylsulfonyl fluoride (PMSF), and halt protease inhibitor cocktail [Thermo Fisher Scientific, USA]) for 1 h. The samples were centrifuged at 12 000 g for 10 min at 4°C, and the supernatants were collected. .. The proteins were fractionated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and the phosphorylated proteins were analyzed by immunoblotting with anti-phosphoserine (anti-Ser) and anti-phosphothreonine (anti-Thr) antibodies (Abcam, USA).
Article Title: Griseofulvin Inhibits Root Growth by Targeting Microtubule-Associated Proteins Rather Tubulins in Arabidopsis .
Article Snippet: .. Five-day-old Col-0 seedlings grown on conventional 1/2 MS medium were transferred into liquid 1/2 MS medium with 40 μM griseofulvin or 1% DMSO and incubated for 1 h or into liquid 1/2 MS medium with 20 μM trifluralin for 10 min. Total RNA from the treated seedlings was extracted using TRIzol reagent according to the manufacturer’s protocol (Invitrogen, Eugene, OR, USA). .. RNA was converted to cDNA using PrimeScript RT reagent Kit with gDNA Eraser (RR047A, TaKaRa, Tokyo, Japan) according to the manufacturer’s instructions.
Article Title: Griseofulvin Inhibits Root Growth by Targeting Microtubule-Associated Proteins Rather Tubulins in Arabidopsis
Article Snippet: .. Five-day-old Col-0 seedlings grown on conventional 1/2 MS medium were transferred into liquid 1/2 MS medium with 40 μM griseofulvin or 1% DMSO and incubated for 1 h or into liquid 1/2 MS medium with 20 μM trifluralin for 10 min. Total RNA from the treated seedlings was extracted using TRIzol reagent according to the manufacturer’s protocol (Invitrogen, Eugene, OR, USA). .. RNA was converted to cDNA using PrimeScript RT reagent Kit with gDNA Eraser (RR047A, TaKaRa, Tokyo, Japan) according to the manufacturer’s instructions.
Protein Extraction:Article Title: Endophytic commensal bacteria capitalize on the AvrPto-FER pathway to enhance proliferation during early stages of pathogen invasion.
Article Snippet: .. The bacterial suspension was adjusted to OD600 = 0.1, and Col-0 seedlings were incubated in the bacterial suspension for 8 h. The seedlings were ground and subjected to protein extraction in extraction buffer (20 mM HEPES pH 7.5, 150 mM NaCl, 10% [v/v] glycerol, 1% [v/v] Triton X-100, 5 mM EDTA, 10 mM DTT, 1 mM PMSF, and Halt Protease Inhibitor Cocktail [Thermo Fisher Scientific, USA]) for 1 h. The samples were centrifuged at 12,000 g for 10 min at 4°C, and the supernatants were collected. .. The samples were run on a 10% SDS-PAGE gel and analyzed by immunoblotting with anti-FER and anti-FERpY648 antibodies (ABclonal, China).
Article Title: Endophytic commensal bacteria capitalize on the AvrPto-FER pathway to enhance proliferation during early stages of pathogen invasion
Article Snippet: .. The bacterial suspension was adjusted to OD 600 = 0.1 and Col-0 seedlings were incubated in the bacterial suspension for 8 h. The seedlings were ground and subjected to protein extraction in extraction buffer (20 mM HEPES pH 7.5, 150 mM NaCl, 10% [v/v] glycerol, 1% [v/v] Triton X-100, 5 mM EDTA, 10 mM DTT, 1 mM phenylmethylsulfonyl fluoride (PMSF), and halt protease inhibitor cocktail [Thermo Fisher Scientific, USA]) for 1 h. The samples were centrifuged at 12 000 g for 10 min at 4°C, and the supernatants were collected. .. The proteins were fractionated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and the phosphorylated proteins were analyzed by immunoblotting with anti-phosphoserine (anti-Ser) and anti-phosphothreonine (anti-Thr) antibodies (Abcam, USA).
Extraction:Article Title: Endophytic commensal bacteria capitalize on the AvrPto-FER pathway to enhance proliferation during early stages of pathogen invasion.
Article Snippet: .. The bacterial suspension was adjusted to OD600 = 0.1, and Col-0 seedlings were incubated in the bacterial suspension for 8 h. The seedlings were ground and subjected to protein extraction in extraction buffer (20 mM HEPES pH 7.5, 150 mM NaCl, 10% [v/v] glycerol, 1% [v/v] Triton X-100, 5 mM EDTA, 10 mM DTT, 1 mM PMSF, and Halt Protease Inhibitor Cocktail [Thermo Fisher Scientific, USA]) for 1 h. The samples were centrifuged at 12,000 g for 10 min at 4°C, and the supernatants were collected. .. The samples were run on a 10% SDS-PAGE gel and analyzed by immunoblotting with anti-FER and anti-FERpY648 antibodies (ABclonal, China).
Article Title: Endophytic commensal bacteria capitalize on the AvrPto-FER pathway to enhance proliferation during early stages of pathogen invasion
Article Snippet: .. The bacterial suspension was adjusted to OD 600 = 0.1 and Col-0 seedlings were incubated in the bacterial suspension for 8 h. The seedlings were ground and subjected to protein extraction in extraction buffer (20 mM HEPES pH 7.5, 150 mM NaCl, 10% [v/v] glycerol, 1% [v/v] Triton X-100, 5 mM EDTA, 10 mM DTT, 1 mM phenylmethylsulfonyl fluoride (PMSF), and halt protease inhibitor cocktail [Thermo Fisher Scientific, USA]) for 1 h. The samples were centrifuged at 12 000 g for 10 min at 4°C, and the supernatants were collected. .. The proteins were fractionated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and the phosphorylated proteins were analyzed by immunoblotting with anti-phosphoserine (anti-Ser) and anti-phosphothreonine (anti-Thr) antibodies (Abcam, USA).
Protease Inhibitor:Article Title: Endophytic commensal bacteria capitalize on the AvrPto-FER pathway to enhance proliferation during early stages of pathogen invasion.
Article Snippet: .. The bacterial suspension was adjusted to OD600 = 0.1, and Col-0 seedlings were incubated in the bacterial suspension for 8 h. The seedlings were ground and subjected to protein extraction in extraction buffer (20 mM HEPES pH 7.5, 150 mM NaCl, 10% [v/v] glycerol, 1% [v/v] Triton X-100, 5 mM EDTA, 10 mM DTT, 1 mM PMSF, and Halt Protease Inhibitor Cocktail [Thermo Fisher Scientific, USA]) for 1 h. The samples were centrifuged at 12,000 g for 10 min at 4°C, and the supernatants were collected. .. The samples were run on a 10% SDS-PAGE gel and analyzed by immunoblotting with anti-FER and anti-FERpY648 antibodies (ABclonal, China).
Article Title: Endophytic commensal bacteria capitalize on the AvrPto-FER pathway to enhance proliferation during early stages of pathogen invasion
Article Snippet: .. The bacterial suspension was adjusted to OD 600 = 0.1 and Col-0 seedlings were incubated in the bacterial suspension for 8 h. The seedlings were ground and subjected to protein extraction in extraction buffer (20 mM HEPES pH 7.5, 150 mM NaCl, 10% [v/v] glycerol, 1% [v/v] Triton X-100, 5 mM EDTA, 10 mM DTT, 1 mM phenylmethylsulfonyl fluoride (PMSF), and halt protease inhibitor cocktail [Thermo Fisher Scientific, USA]) for 1 h. The samples were centrifuged at 12 000 g for 10 min at 4°C, and the supernatants were collected. .. The proteins were fractionated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and the phosphorylated proteins were analyzed by immunoblotting with anti-phosphoserine (anti-Ser) and anti-phosphothreonine (anti-Thr) antibodies (Abcam, USA).
Saline:Article Title: Reference genes for quantitative Arabidopsis single molecule RNA fluorescence in situ hybridization
Article Snippet: After 2 d of stratification at 4 °C, the plates were then positioned upright in a PHCBI growth chamber (model MLR-352H-PE, PHC Corporation, Japan) set to 16-h light–8-h dark cycles at a constant 20 °C. .. Briefly, 5-day-old Col-0 seedlings were fixed in 16% methanol-free formaldehyde diluted to 4% in 1× phosphate-buffered saline (PBS; Thermo Fisher Scientific, Waltham, MA, USA) for 30 min then washed three times with 1× PBS. .. Briefly, 5-day-old Col-0 seedlings were fixed in 16% methanol-free formaldehyde diluted to 4% in 1× phosphate-buffered saline (PBS; Thermo Fisher Scientific, Waltham, MA, USA) for 30 min then washed three times with 1× PBS.
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